Lasers and Filters in the 4 Laser FACS Aria III

Lasers

Longpass Filters

Bandpass Filters

Examples of appropriate fluorochromes

405 nm

50 mW

-

450/40

V450, Brilliant Violet 421, Pacific Blue, Alexa Fluor® 405, DAPI

 

502

510/50

V500, AmCyan, Briliant Violet 510, Alexa 430

570605/30 Brilliant Violet 605, Qdot 605
     

488 nm

20 mW

502

530/30

FITC, GFP, CFSE, Alexa Fluor 488

  655

695/40

PerCP-CyTM5.5, PerCP

     

561 nm

50 mW

-

582/15

PE, AsRed, tdTomato

 

600

610/20

PE-Texas Red®, Living Colors® mCherry, PI

  630

670/14

PE-CyTM5, 7-AAD

  735

780/60

PE-CyTM7

     

633 nm

20 mW

-

660/20

APC, Alexa Flour® 647

  690

730/45

Alexa Fluor® 700, Alexa 680

  735

780/60

APC-Cy7, APC-H7, nIR Live /Dead