Lasers and Filters in the 3 Laser FACS Aria III

Lasers

Longpass Filters

Bandpass Filters

Examples of appropriate fluorochromes

375 nm 

15  mW

-

450/50

V450, Hoechst Blue, DAPI, Brilliant Violet 421

 

502

510/50

V500, AmCyan

488 nm

20 mW

502

530/30

FITC, GFP, CFSE, Alexa 488

 

556

585/42

PE

 

610

616/23

PE-Texas Red®,  PI

 

655

695/40

PerCP-CyTM5.5, PerCP, 7-AAD

 

735

780/60

PE-CyTM7

633 nm

20 mW

-

660/20

APC, Alexa 647

 

690

730/45

Alexa Flour® 700, Alexa 680

 

735

780/60

APC-Cy7, APC-H7