Lasers and Filters in the 4 Laser LSRFortessa.

Lasers

Longpass filters

Bandpass filters

Examples of appropriate fluorochromes

405 nm

 

442/46

V450, Brilliant Violet 421, Alexa Fluor® 405, DAPI

 

505 LP

525/50

V500, Brilliant Violet 510, AmCyan, Alexa 430

 

600 LP

610/20

Brilliant Violet 605, Qdot 605

 

650 LP

660/20

Brilliant Violet 650

 

685 LP

711/25

Brilliant Violet 711

 

750 LP

785/60

Brilliant Violet 785

 

 

 

 

488 nm

505 LP

530/30

FITC, GFP, CFSE, Alexa Fluor 488

685 LP

710/50

PerCP, PerCP-CyTM5.5

561 nm

570 LP

586/15

PE, AsRed, td Tomato

600 LP

615/20

PE-Texas Red®, Living Colors® mCherry, PI

 

635 LP

660/20

PE-CyTM5, 7-AAD

685 LP

710/40

PE-CyTM5.5

750 LP

780/60

PE-CyTM7

 

 

 

 

640 nm

 

670/30

APC, Alexa Flour® 647

690 LP

730/45

Alexa Fluor® 700, Alexa 680

750 LP

780/60

APC-Cy7, APC-H7, nIR Live/Dead