Lasers and Filters in the 4 Laser FACS Aria III

Lasers

Filters

Examples of appropriate fluorochromes

405 nm

450/50

V450, Brilliant Violet 421, Pacific Blue, Alexa Fluor® 405, DAPI

 

510/50

V500, AmCyan, Briliant Violet 510, Alexa 430

605/30 Brilliant Violet 605, Qdot 605

 

 

 

488 nm

530/30

FITC, GFP, CFSE, Alexa Fluor 488

 

695/40

PerCP-CyTM5.5, PerCP

 

 

 

561 nm

582/15

PE, AsRed, tdTomato

 

610/20

PE-Texas Red®, Living Colors® mCherry, PI

 

670/14

PE-CyTM5, 7-AAD

 

780/60

PE-CyTM7

 

 

 

633 nm

660/20

APC, Alexa Flour® 647

 

730/45

Alexa Fluor® 700, Alexa 680

 

780/60

APC-Cy7, APC-H7, nIR Live /Dead