Lasers and Filters in the 3 Laser FACS Aria III

Lasers

Filters

Examples of appropriate fluorochromes

375 nm

450/50

V450, Hoechst Blue, DAPI, Brilliant Violet 421

 

510/50

V500, AmCyan

488 nm

530/30

FITC, GFP, CFSE, Alexa 488

 

585/42

PE

 

616/23

PE-Texas Red®,  PI

 

695/40

PerCP-CyTM5.5, PerCP, 7-AAD

 

780/60

PE-CyTM7

633 nm

660/20

APC, Alexa 647

 

730/45

Alexa Flour® 700, Alexa 680

 

780/60

APC-Cy7, APC-H7